The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 18, 2020
Filed:
Oct. 17, 2014
Bgi Shenzhen, Shenzhen, CN;
Chunyu Geng, Shenzhen, CN;
Hongyan Han, Shenzhen, CN;
Guangying Guo, Shenzhen, CN;
Wenwei Zhang, Shenzhen, CN;
Hui Jiang, Shenzhen, CN;
Yuan Jiang, Shenzhen, CN;
MGI TECH CO., LTD, Shenzhen, Guangdong, CN;
Abstract
The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5'-X-Y-3′, and the sequence composition of the downstream random primers is 5′-P-Y′-X′-close-3′, wherein Y and Y′ are random sequences, X is all or part of sequences of a sequencing platform 5′ end adaptor, X′ is all or part of sequences of a sequencing platform 3′ end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken.