The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 28, 2020
Filed:
Jan. 05, 2018
The United States of America, As Represented BY the Secretary of Agriculture, Washington, DC (US);
University of Maryland, College Park, College Park, MD (US);
Western University of Health Sciences, Pomona, CA (US);
David M. Donovan, Essex, MD (US);
Jerel Waters, Randallstown, MD (US);
Dayana T. Rowley, Beltsville, MD (US);
Steven M. Swift, Bethesda, MD (US);
Brian B. Oakley, Claremont, CA (US);
The United States of America, as Represented by the Secretary of Agriculture, Washington, DC (US);
Western University of Health Sciences, Pamona, CA (US);
University of Maryland, College Park, College Park, MD (US);
Abstract
can cause food poisoning and is a major agent in necrotic enteritis. As laws banning the use of antibiotics in animal feed become more common, the need for alternatives to antibiotics becomes greater. Peptidoglycan hydrolases that target the cell wall of specific bacteria are one such alternative. Genes for four endolysins, PlyCP10, PlyCP18, PlyCP33, and PlyCP41, were found within clusters of phage associated genes, likely prophages from strains Cp10, Cp18, Cp33, and Cp41. PlyCP18 and PlyCP33 harbor L-alanine amidase catalytic domains, and PlyCP10 and PlyCP41 have glycosyl hydrolase catalytic domains as predicted by BlastP and PFAM searches. All four genes were synthesized withcodon optimization, expressed inexpression vectors with a 6×His tag for nickel column purification, and the recombinant proteins purified. The four endolysins were capable of lysing the 66strains tested but not the other bacteria tested.