The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 31, 2019

Filed:

Nov. 24, 2018
Applicant:

Alfaisal University, Riyadh, SA;

Inventors:

Shimaa Eissa, Riyadh, SA;

Mohammed Zourob, Riyadh, SA;

Raja Chinnappan, Riyadh, SA;

Ayesha Siddiqua, Riyadh, SA;

Assignee:

Alfaisal University, Riyadh, SA;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); G01N 27/327 (2006.01); C12Q 1/686 (2018.01); C12Q 1/6806 (2018.01); C12N 15/115 (2010.01); G16B 30/00 (2019.01);
U.S. Cl.
CPC ...
G01N 27/3275 (2013.01); C12N 15/115 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6806 (2013.01); G01N 27/3273 (2013.01); G16B 30/00 (2019.02);
Abstract

An electrochemical screening method for the selection of DNA aptamers against 11-deoxycortisol (11-DCL) using gold electrode for target immobilization is described. The gold electrode is used as solid matrix instead of the beads for SELEX. The selection steps (SELEX) are performed on the 11-DC modified electrode directly as the DNA library in the first round or the enriched DNA pools in the subsequent rounds were incubated on the electrode, then the unbound DNA is washed and the bound DNA is measured directly by square wave voltammetry. Then elution of the bound DNA is performed for further use.


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