The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 24, 2019
Filed:
May. 06, 2016
Applicant:
Affymetrix, Inc., Santa Clara, CA (US);
Inventors:
Yuker Wang, Palo Alto, CA (US);
Keith W. Jones, Sunnyvale, CA (US);
Ronald J. Sapolsky, Palo Alto, CA (US);
Assignee:
Other;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12P 19/28 (2006.01); C12Q 1/6811 (2018.01); C12Q 1/6832 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/68 (2018.01); C12Q 1/6876 (2018.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12N 15/1093 (2013.01); C12P 19/28 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6811 (2013.01); C12Q 1/6832 (2013.01); C12Q 1/6876 (2013.01);
Abstract
Compositions, methods and kits are disclosed for synthesizing and amplifying pools of probes using precursor oligonucleotides. In some aspects the precursor is amplified and nicking enzymes are used to separate the full length probes from the amplification products. The methods enable the preparation of single stranded DNA probes of defined sequence and length that are suitable for use in target detection assays.