The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 17, 2019
Filed:
Feb. 08, 2018
Recombinantfor high efficiency production of fructosylated chondroitin and method for making thereof
Jing Wu, Wuxi, CN;
Quan Zhang, Wuxi, CN;
Liming Liu, Wuxi, CN;
Xiulai Chen, Wuxi, CN;
Jia Liu, Wuxi, CN;
Qiuling Luo, Wuxi, CN;
Jing Wu, Wuxi, CN;
Quan Zhang, Wuxi, CN;
Liming Liu, Wuxi, CN;
Xiulai Chen, Wuxi, CN;
Jia Liu, Wuxi, CN;
Qiuling Luo, Wuxi, CN;
Jiangnan University, Wuxi, JS, CN;
Abstract
The present invention relates to the field of biotechnology engineering. It provides a recombinantthat can produce fructosylated chondroitin with high yields and the method for constructing the recombinant strain. The present invention discloses a method for constructing an expression plasmid pETM6R1-RBS-glmM-GGGS-glmS that contains a glmM and a glmS gene under regulation of ribosome binding sites. The recombinant plasmid was transformed intoK4 to obtain a recombinantstrain ZQ33 that can produce fructosylated chondroitin up to 3.99 g·Lby fed-batch fermentation in a 5-L fermentor, which was increased by 108.90% compared to that of the wild type strain.