The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 10, 2019

Filed:

Mar. 21, 2016
Applicant:

The Chinese University of Hong Kong, Shatin, CN;

Inventors:

Jiawei Liao, Tai Po Market, CN;

Kwan Chee Chan, Shatin, CN;

Wai Kwun Rossa Chiu, Shatin, CN;

Yuk Ming Dennis Lo, Homantin, CN;

Assignee:

The Chinese University of Hong Kong, Shatin, New Territories, HK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6883 (2018.01); G16B 25/00 (2019.01); G16B 30/00 (2019.01); G16B 20/40 (2019.01); C12Q 1/6827 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6883 (2013.01); C12Q 1/6827 (2013.01); G16B 20/40 (2019.02); G16B 25/00 (2019.02); G16B 30/00 (2019.02); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01);
Abstract

Whether a fetus has an aneuploidy associated with a first chromosome is detected using ratios of alleles detected in a maternal sample having a mixture of maternal and fetal DNA. DNA from the sample is enriched for target regions associated with polymorphic loci and then sequenced. Polymorphic loci (e.g., single nucleotide polymorphisms) in the target regions with fetal-specific alleles are identified on a first chromosome and on one or more reference chromosomes. A first ratio of the fetal-specific alleles and shared alleles is determined for the loci on the first chromosome. A second ratio of the fetal-specific alleles and shared alleles is determined for the loci on the reference chromosome(s). A third ratio of the first and second ratio can be compared to a cutoff to determine whether an aneuploidy is present, and whether the aneuploidy is maternally-derived or paternally-derived.


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