The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 26, 2019

Filed:

Nov. 30, 2018
Applicant:

Mitsubishi Chemical Corporation, Tokyo, JP;

Inventors:

Fumiaki Watanabe, Kanagawa, JP;

Ai Hara, Kanagawa, JP;

Takanori Ambo, Aichi, JP;

Aya Kitahara, Kanagawa, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/88 (2006.01); C07K 14/47 (2006.01); C12P 13/02 (2006.01);
U.S. Cl.
CPC ...
C12N 9/88 (2013.01); C07K 14/472 (2013.01); C12P 13/02 (2013.01); C12Y 402/01084 (2013.01);
Abstract

Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GXXXXDXXR) in a beta subunit, and is characterized in that Xis an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.


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