The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 2019

Filed:

Jun. 15, 2018
Applicant:

Hoffmann-la Roche Inc., Little Falls, NJ (US);

Inventors:

Gregor Jordan, Groebenzell, DE;

Roland Staack, Munich, DE;

Assignee:

Hoffmann-La Roche Inc., Little Falls, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 31/00 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/536 (2006.01); G01N 33/68 (2006.01); G01N 33/94 (2006.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54306 (2013.01); G01N 33/536 (2013.01); G01N 33/58 (2013.01); G01N 33/6854 (2013.01); G01N 33/94 (2013.01);
Abstract

Herein is reported a method for determining in a sample the (total, i.e. binding competent) amount of a ligand of a ligand-binding protein (therapeutic) comprising the following steps in the following order: subjecting the sample to an acid treatment, forming in solution a non-covalent complex comprising i) an anti-ligand antibody, ii) the ligand, and iii) labelled ligand-binding protein, by adding anti-ligand antibody and labelled ligand-binding protein to the sample, and determining the amount of the complex and thereby determining the amount of the ligand of the ligand-binding protein.


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