The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 2019

Filed:

Oct. 22, 2014
Applicant:

Hitachi High-technologies Corporation, Tokyo, JP;

Inventors:

Masako Kawarai, Tokyo, JP;

Toshinari Sakurai, Tokyo, JP;

Akihiro Shimase, Tokyo, JP;

Hiroyuki Koshi, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01J 19/00 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12M 1/00 (2006.01); G01N 15/06 (2006.01); G01N 21/53 (2006.01); G01N 33/483 (2006.01); G01N 33/50 (2006.01); G01N 15/00 (2006.01);
U.S. Cl.
CPC ...
B01J 19/0006 (2013.01); C12M 41/46 (2013.01); C12M 41/48 (2013.01); C12M 47/02 (2013.01); C12M 47/04 (2013.01); G01N 15/06 (2013.01); G01N 21/53 (2013.01); G01N 33/4833 (2013.01); G01N 33/5005 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/0693 (2013.01); G01N 2201/127 (2013.01);
Abstract

A cytometric mechanism includes: a flow path through which a cell suspension is made to flow; a liquid drive unit for sending the cell suspension which is in the flow path; and a computation unit for irradiating, with irradiation light from a light source, a cell suspension flowing through a flow cell, and for finding a cell survival rate in the cell suspension on the basis of a resulting forward scattered light intensity and transmittance and/or side scattered light intensity. The invention is provided with a calibration curve database for storing, in advance, respective calibration curves indicative of a relationship between viable cell concentration and forward scattered light intensity, a relationship between dead cell concentration and the transmittance, and a relationship between a cell survival rate and the side scattered light intensity.


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