The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 2019
Filed:
Apr. 25, 2016
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Ira Schildkraut, Boxford, MA (US);
Laurence Ettwiller, Beverly, MA (US);
Ivan R. Correa, Jr., Ipswich, MA (US);
Michael Sproviero, Ipswich, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6806 (2018.01); C12N 15/10 (2006.01); C12Q 1/6869 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12N 15/1003 (2013.01); C12N 15/1034 (2013.01); C12Q 1/6869 (2013.01);
Abstract
A method of enriching for a population of RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding an affinity tag to the 5' end of 5′-diphosphorylated or 5′-triphosphorylated RNA molecules in a sample by incubating the sample with an affinity tag-labeled GTP and a capping enzyme; and (b) enriching for RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag.