The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 10, 2019
Filed:
Feb. 04, 2015
Institut Pasteur, Paris, FR;
Centre National DE LA Recherche Scientifique, Paris, FR;
Institut National DE LA Sante ET DE LA Recherche Medicale, Paris, FR;
Pierre Charneau, Paris, FR;
Veronique Zennou, New York, NY (US);
Francoise Pflumio, Vitry-sur-Seine, FR;
Aude Sirven, Paris, FR;
Anne Dubart Kupperschmitt, Choisy-le-Roi, FR;
Institut Pasteur, Paris, FR;
Institut National de la Santé et de la Recherche Médicale, Paris, FR;
Centre National de la Recherche Scientifique, Paris, FR;
Abstract
The present invention provides nucleic acid, vectors, viruses, and recombinant cells comprising triple-stranded structures, such as those resulting from central initiation and termination of HIV-1 reverse transcription at the center of HIV-1 linear DNA genomes. These triplex structures can act as a cis-determinant of HIV-1 DNA nuclear import, allowing infection of non-dividing target cells. In one aspect, the presence of the DNA triplex sequence in an HIV vector strongly stimulates gene transfer in hematopoietic stem cells. The invention also provides methods of using these triplex structures for making recombinant cells, as well as methods of using the recombinant cells to express proteins of interest both in vitro and in vivo.