The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 03, 2019

Filed:

Nov. 10, 2015
Applicant:

Nanjing Bestzyme Bio-engineering Co., Ltd., Nanjing, CN;

Inventors:

Yan Fan, Nanjing, CN;

Yamin Lu, Nanjing, CN;

Xiuzhen Du, Nanjing, CN;

Huadong Du, Nanjing, CN;

Feng Li, Nanjing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/02 (2006.01); C07K 14/32 (2006.01); C12N 9/44 (2006.01); C12P 19/04 (2006.01); C12P 19/14 (2006.01); C12P 19/02 (2006.01); C12P 19/16 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2457 (2013.01); C12P 19/02 (2013.01); C12P 19/04 (2013.01); C12P 19/14 (2013.01); C12P 19/16 (2013.01); C12Y 302/01041 (2013.01);
Abstract

Truncated pullulanases having an N-terminal deletion in a parental pullulanase are provided. These truncated pullulanases have altered properties as compared to the parental pullulanase, including an increased saccharification rate and higher catalytic activity at acidic pH values below 4.5 and higher temperatures of up to 64° C. These truncated pullulanase also have improved thermal stability as compared to the parental enzyme. Also provided are compositions containing the truncated pullulanases and a glucoamylase, and processes for applying the truncated pullulanases and compositions in the starch industry. Polynucleotides that encode the truncated pullulanases, and recombinant host cells for producing the truncated pullulanases are also described.


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