The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 20, 2019
Filed:
Apr. 18, 2016
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Walter Lee, Campbell, CA (US);
Tyson Bowen, Union City, CA (US);
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Abstract
Methods for adding sequence tags during amplification of a nucleic acid target are provided. The target is amplified using two sets of primers that are modified such that only tagged targets can ligate to hairpin adapters that protect them from subsequent exonuclease treatment. Methods of making circular nucleic acids are also provided. Specific overhangs are created by exonuclease trimming with a polymerase such that only desired targets can ligate to sticky ended hairpin adapters that protect them from subsequent exonuclease treatment. Compositions, kits, and systems related to or useful in the methods are also described.