The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 16, 2019

Filed:

Aug. 09, 2013
Applicant:

National Institute of Advanced Industrial Science and Technology, Tokyo, JP;

Inventors:

Hisashi Narimatsu, Tsukuba, JP;

Atsushi Kuno, Tsukuba, JP;

Yuzuru Ikehara, Tsukuba, JP;

Yasuhiro Hashimoto, Fukushima, JP;

Keiro Shirotani, Fukushima, JP;

Kiyomitsu Nara, Fukushima, JP;

Yoshinobu Kariya, Fukushima, JP;

Hiromi Ito, Fukushima, JP;

Kyoka Hoshi, Fukushima, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); G01N 33/536 (2006.01); G01N 33/53 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5308 (2013.01); G01N 33/536 (2013.01); G01N 33/6854 (2013.01); G01N 2333/42 (2013.01); G01N 2333/4724 (2013.01); G01N 2400/00 (2013.01); G01N 2440/38 (2013.01); G01N 2496/00 (2013.01);
Abstract

It is intended to develop and provide a method for detecting a particular glycan-isoform rapidly and specifically by a small number of steps. The present invention provides a glycan-isoform detection method comprising quantifying an immune complex formed by the mixing of a test sample with a sugar chain non-reducing terminal residue-binding lectin and an antibody specifically binding to the protein moiety of the glycan-isoform, etc., comparing the obtained amount of the immune complex with the amount of a control immune complex obtained when a control sample is not mixed with the sugar chain non-reducing terminal residue-binding lectin or is mixed with a control protein, and determining the presence or absence of the glycan-isoform of interest in the test sample on the basis of the difference between these amounts.


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