The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 16, 2019

Filed:

Nov. 11, 2014
Applicant:

Agency for Science, Technology and Research, Singapore, SG;

Inventors:

Daniele Zink, Singapore, SG;

Jackie Y. Ying, Singapore, SG;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/071 (2010.01); A61K 35/545 (2015.01); A61K 35/22 (2015.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01); A61L 27/54 (2006.01); A61L 27/58 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0686 (2013.01); A61K 35/22 (2013.01); A61K 35/545 (2013.01); A61L 27/3604 (2013.01); A61L 27/3641 (2013.01); A61L 27/3839 (2013.01); A61L 27/3895 (2013.01); A61L 27/54 (2013.01); A61L 27/58 (2013.01); A61L 2300/414 (2013.01); A61L 2300/64 (2013.01); A61L 2430/26 (2013.01); A61L 2430/40 (2013.01); C12N 2501/155 (2013.01); C12N 2501/727 (2013.01); C12N 2506/45 (2013.01); C12N 2533/90 (2013.01);
Abstract

There is provided a method of differentiating an induced pluripotent stem cell (iPSC) into a renal proximal tubular cell (PTC)-like cell. The method comprises culturing an undifferentiated iPSC in a renal epithelial cell culture medium in the presence of one or more extracellular matrix (ECM) molecules, bone morphogenic protein 2 (BMP2) and bone morphogenic protein 7 (BMP7), for a period of from about 8 to about 10 days, under conditions sufficient to induce differentiation of the iPSC into a PTC-like cell. A cell population of differentiated PTC-like cells is also provided, as well as uses and methods of use of the cell population.


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