The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 25, 2019

Filed:

May. 11, 2015
Applicant:

Echelon Diagnostics, Inc., Reno, NV (US);

Inventors:

John Burke, Reno, NV (US);

Stephen Healy Sanders, Reno, NV (US);

Assignee:

ECHELON DIAGNOSTICS, INC., Reno, NV (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G16B 30/10 (2019.01); G06F 19/22 (2011.01);
U.S. Cl.
CPC ...
G06F 19/22 (2013.01);
Abstract

Techniques for construction of internal controls for improved accuracy and sensitivity of DNA testing include obtaining first data and determining weights over real numbers for a normalization function in less than a day. The first data indicates a measured amount of reference sequences for nucleic acids from training samples. The reference sequences include a target, for which an abundance is indicative of a condition of interest, and covariates not correlated with the condition of interest. The normalization function involves a sum of abundances of the covariates, as internal controls, each multiplied by a corresponding one of the weights. The weights are determined based on minimizing variance of a Taylor expansion of a ratio of a measured amount of the target divided by a value of the normalization function evaluated with measured amounts of the covariates over a portion of the first data in which the condition is absent.


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