The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 25, 2019

Filed:

Feb. 22, 2016
Applicant:

Adimab, Llc, Lebanon, NH (US);

Inventors:

Li Zhu, Burbank, CA (US);

Shuanghong Wei, Cupertino, CA (US);

Shaobing B. Hua, Cupertino, CA (US);

Assignee:

ADIMAB, LLC, Lebanon, NH (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C40B 30/04 (2006.01); C12N 15/10 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1041 (2013.01); C12N 15/1037 (2013.01); C12N 15/1062 (2013.01); C12N 15/1086 (2013.01); G01N 33/6845 (2013.01); G01N 33/6854 (2013.01); C40B 30/04 (2013.01);
Abstract

Compositions, methods, and kits are provided for efficiently generating and screening humanized antibody with high affinity against a specific antigen. The library of humanized antibody is generated by mutagenizing a chimeric antibody template that combines human antibody framework and antigen binding sites of a non-human antibody. Alternatively, the library of humanized antibody is generated by grafting essential antigen-recognition segment(s) such as CDRs of the non-human antibody into the corresponding position(s) of each member of a human antibody library. This library of humanized antibody is then screened for high affinity binding toward a specific antigen in vivo in organism such as yeast or in vitro using techniques such as ribosome display or mRNA display. The overall process can be efficiently performed in a high throughput and automated manner, thus mimicking the natural process of antibody affinity maturation.


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