The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 28, 2019

Filed:

Nov. 18, 2016
Applicant:

University of Florida Research Foundation, Inc., Gainesville, FL (US);

Inventor:

Yunwei Charles Cao, Gainesville, FL (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12Q 1/6816 (2018.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01); B01L 7/00 (2006.01); G01N 35/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6816 (2013.01); B01L 3/502761 (2013.01); G01N 33/54313 (2013.01); G01N 33/587 (2013.01); B01L 7/00 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0636 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/10 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0424 (2013.01); B01L 2400/0487 (2013.01); G01N 35/1097 (2013.01);
Abstract

Described herein are fluid-manipulation-based devices. Fluid manipulations as described herein can be configured to perform assays on biological samples. In an embodiment, the device includes a reaction chamber, which can includes an integrated sample isolation module, a cell lysis module, a biological target purification module, and an assay mixing module, which can include a microbead with a capture molecule coupled thereto and a nanoparticle having a probe molecule coupled thereto via a label, which can be a spectroscopic label. In an embodiment, the capture and probe molecules can be configured to be coupled together via a biological target to form a biological molecule bead complex. Devices and methods as described herein can manipulate and analyze nanoliter volumes of fluid, microliter volumes of fluid, milliliter volumes of fluid, or greater. Embodiments of the present disclosure can enable random biological assays and rapid, simultaneous analysis of multiple biological samples.


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