The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 21, 2019

Filed:

Jan. 17, 2014
Applicants:

Daniel Zabetakis, Brandywine, MD (US);

George P. Anderson, Bowie, MD (US);

Ellen R. Goldman, Germantown, MD (US);

Kendrick Turner, Washington, DC (US);

P. Audrey Brozozog Lee, Washington, DC (US);

Inventors:

Daniel Zabetakis, Brandywine, MD (US);

George P. Anderson, Bowie, MD (US);

Ellen R. Goldman, Germantown, MD (US);

Kendrick Turner, Washington, DC (US);

P. Audrey Brozozog Lee, Washington, DC (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 14/47 (2006.01); C07K 16/12 (2006.01); C07K 16/16 (2006.01); C12N 5/06 (2006.01);
U.S. Cl.
CPC ...
C07K 14/47 (2013.01); C07K 16/1271 (2013.01); C07K 16/1278 (2013.01); C07K 16/16 (2013.01); C07K 2317/569 (2013.01); C07K 2317/94 (2013.01); C07K 2319/00 (2013.01);
Abstract

Methods of producing a single-domain antibody (sdAb) include causing a bacteria to express the sdAb into cytoplasm of the bacteria, wherein the sdAb is expressed as a fusion protein with the acid tail of α-synuclein; and then purifying the sdAb, wherein the fusion protein is expressed free of a periplasmic location tag. Such antibodies have the unexpected ability to refold after thermal denaturation.


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