The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 30, 2019

Filed:

Dec. 06, 2013
Applicant:

The Curators of the University of Missouri, Columbia, MO (US);

Inventors:

Shramik Sengupta, Columbia, MO (US);

Sachidevi Puttaswamy, Columbia, MO (US);

Hsueh-Chia Chang, Granger, IN (US);

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); C12Q 1/06 (2006.01); C12Q 1/04 (2006.01); G01N 33/543 (2006.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/06 (2013.01); C12Q 1/04 (2013.01); G01N 33/5438 (2013.01); B01L 3/502715 (2013.01);
Abstract

An improved system and method is provided for detecting viable bacteria in a suspension sample. A sample of a suspension in which bacterial presence is suspected is collected from a source and a portion of the sample transferred to a microfluidic unit. A series of analysis signals at different frequencies are applied to the sample portion. An impedance is measured via a signal analyzer for the sample portion for each of the analysis signals to define an impedance data set. An initial bulk capacitance value is determined for a model circuit based on the impedance dataset. After a predetermined time period, a new bulk capacitance value is determined for on another portion of the sample. The difference between the new bulk capacitance and the initial bulk capacitance value is compared to a threshold value to determine if viable bacterial is present in the sample.


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