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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10273519 B1

PDFFull Text
Date of Patent:
Apr. 30, 2019

Filed:

Jul. 23, 2014

Diterpene production in yarrowia

Applicant:

Dsm Ip Assets B.v., Heerlen, NL;

Inventors:

Nicolette Jasmijn Broers, Echt, NL;

Viktor Marius Boer, Echt, NL;

Adam G. Lawrence, Lexington, MA (US);

Assignee:

DSM IP ASSETS B.V., Heerlen, NL;

Attorneys:

McBee Moore Woodward & Vanik IP, LLC

Susan McBee

Chester Moore

Primary Examiner:

Suzanne M Noakes

Int. Cl.
CPC ...
C12P 19/56 (2006.01); A23L 2/60 (2006.01); C12N 9/00 (2006.01); C12N 15/52 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12N 15/00 (2006.01); C07C 62/32 (2006.01); C07H 15/256 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12P 15/00 (2006.01); A23L 27/30 (2016.01);
U.S. Cl.
CPC ...
C12P 19/56 (2013.01); A23L 2/60 (2013.01); A23L 27/36 (2016.08); C07C 62/32 (2013.01); C07H 15/256 (2013.01); C12N 9/0042 (2013.01); C12N 9/0073 (2013.01); C12N 9/0083 (2013.01); C12N 9/1051 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12P 15/00 (2013.01); C07C 2603/86 (2017.05); C12Y 114/13078 (2013.01); C12Y 114/13079 (2013.01); C12Y 402/03019 (2013.01); C12Y 505/01013 (2013.01);
Abstract

The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism of the genusin a suitable fermentation medium at a temperature of about 29° C. or higher, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol; and b. recovering the diterpene or glycosylated diterpene.

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