The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 09, 2019

Filed:

Sep. 23, 2016
Applicant:

The United States of America, As Represented BY the Secretary of Agriculture, Washington, DC (US);

Inventor:

Michael L Sullivan, Madison, WI (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); C12N 9/10 (2006.01); A01H 1/04 (2006.01); C12Q 1/6895 (2018.01);
U.S. Cl.
CPC ...
C12N 15/8243 (2013.01); A01H 1/04 (2013.01); C12N 9/1029 (2013.01); C12N 15/8251 (2013.01); C12Q 1/6895 (2013.01); C12Y 203/01 (2013.01); C12Q 2600/158 (2013.01);
Abstract

Two novel cDNAs for two different genes, HDT1 and HDT2, are isolated from red clover and sequenced. Both HDT1 and HDT2 encode hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase (HDT) which enzymatically produces clovamide and/or related hydroxycinnamoyl amides. Clovamide and related hydroxycinnamoyl amides reduce post-harvest protein degradation. Genetically altered alfalfa plants containing an expression cassette containing a cDNA encoding HDT1 or HDT2 are generated. These genetically altered alfalfa plants produce hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase, which in turn produces clovamide and/or related hydroxycinnamoyl amides.


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