The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 26, 2019
Filed:
Jun. 11, 2014
Applicant:
Kyoto University, Kyoto-shi, Kyoto, JP;
Inventors:
Assignee:
KYOTO UNIVERSITY, Kyoto-Shi, Kyoto, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/074 (2010.01); C12N 15/86 (2006.01); C12N 5/0789 (2010.01); G01N 33/569 (2006.01); C12Q 1/6881 (2018.01); C07K 14/65 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0647 (2013.01); C07K 14/65 (2013.01); C12N 5/0696 (2013.01); C12Q 1/6881 (2013.01); G01N 33/56966 (2013.01); C12N 2501/105 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/14 (2013.01); C12N 2501/145 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/2303 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/2311 (2013.01); C12N 2501/26 (2013.01); C12N 2506/45 (2013.01); C12N 2510/00 (2013.01); C12Q 2600/158 (2013.01);
Abstract
A method for producing hematopoietic stem cells and/or hematopoietic progenitor cells from pluripotent stem cells is described. The method includes a step of culturing pluripotent stem cells in the presence of IGF2. A method is described for selecting an induced pluripotent stem cell(s) having high capacity to differentiate into hematopoietic stem cells and/or hematopoietic progenitor cells, or into blood cells, based on the expression level(s) of one or more genes such as TRIM58, CTSF, FAM19A5, and TCERG1L genes, or on the DNA methylation state(s) of the TRIM58, CSMD1, and/or FAM19A5 gene(s).