The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 22, 2019

Filed:

Dec. 02, 2013
Applicants:

Compagnie Gervais Danone, Paris, FR;

University College Cork—national University of Ireland, Cork, Cork, IE;

Inventors:

Douwe van Sinderen, Carriggrohane, IE;

Mary O'Connell Motherway, Ladysbridge, IE;

Debbie Watson, Mallow, IE;

Tamara Smokvina, Orsay, FR;

Peggy Garault, Montlhery, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); A61K 39/00 (2006.01); A61K 39/38 (2006.01); C12N 15/74 (2006.01); C12N 1/20 (2006.01); C12N 9/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/746 (2013.01); C12N 1/20 (2013.01); C12N 9/1007 (2013.01); C12Y 201/01 (2013.01);
Abstract

The present invention concerns a method for genetically transforming astrain comprising a step of methylation of a shuttle vector in anor a Gram-positive bacterium strain by two type II DNA methyltransferases from a: a methyltransferase enzyme that methylates the adenine base at position 4 of the nucleotide sequence RTCAGG and a methyltransferase enzyme that methylates the cytosine base at position 4 of the nucleotide sequence GGWCC. The present invention also concerns genetic tools and culture media useful for carrying out said method.


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