The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 04, 2018

Filed:

Aug. 12, 2014
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Martin Kampmann, San Francisco, CA (US);

Michael Bassik, San Francisco, CA (US);

Jonathan Weissman, San Francisco, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12Q 1/68 (2018.01); G06F 19/18 (2011.01); G01N 33/50 (2006.01); C12Q 1/6886 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1075 (2013.01); C12N 15/111 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6886 (2013.01); G01N 33/502 (2013.01); G06F 19/18 (2013.01); C12N 2310/14 (2013.01); C12N 2310/531 (2013.01); C12N 2320/12 (2013.01); C12Q 2600/136 (2013.01); C12Q 2600/142 (2013.01);
Abstract

The present invention provides methods for conducting screens using nucleic acid elements (e.g., interfering RNAs) to confidently identify hit genetic elements. The present invention further comprises constructing vectors that contain two or more nucleic acid elements to knock down all pairwise combinations of the hit genetic elements identified from the screen. Following quantitation of the single and double-knockdown phenotypes, genetic interactions between all gene pairs can be calculated. Genes can then be clustered according to the similarity of the pattern of their interactions with all of the other genes to obtain a genetic interaction map, which can advantageously be used to predict functional associations between genes and identify drug targets for therapy such as combination cancer therapy.


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