The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 20, 2018

Filed:

Apr. 23, 2015
Applicant:

Miltenyi Biotec Gmbh, Bergisch Gladbach, DE;

Inventors:

Andrew Kaiser, Rosrath, DE;

Mario Assenmacher, Bergisch Gladbach, DE;

Ian Johnston, Rosrath, DE;

Assignee:

Miltenyi Biotec GmbH, Bergisch Gladbach, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0783 (2010.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0636 (2013.01); C12N 5/0087 (2013.01); C12N 2501/51 (2013.01); C12N 2501/515 (2013.01); C12N 2510/00 (2013.01);
Abstract

The present invention provides a process for generation of genetically modified T cells, T cell subsets and/or T cell progenitors comprising the steps: a) providing a cell sample comprising T cells, T cell subsets and/or T cell progenitors b) preparation of the cell sample by centrifugation c) magnetic separation of the T cells, T cell subsets and/or T cell progenitors d) activation of the enriched T cells, T cell subsets and/or T cell progenitors using modulatory agents e) genetic modification of the T cells, T cell subsets and/or T cell progenitors f) expansion of the genetically modified T cells, T cell subsets and/or T cell progenitors in a cultivation chamber g) washing of the cultured T cells, T cell subsets and/or T cell progenitors characterized in that all steps are performed in a closed and sterile cell culture system.


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