The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 07, 2018
Filed:
Oct. 17, 2006
Carol M. Kinoshita, Bedford, MA (US);
Prashant Mishra, Troy, MI (US);
Marianne Borowski, Winthrop, MA (US);
Peter Francis Daniel, Natick, MA (US);
Carol M. Kinoshita, Bedford, MA (US);
Prashant Mishra, Troy, MI (US);
Marianne Borowski, Winthrop, MA (US);
Peter Francis Daniel, Natick, MA (US);
SHIRE HUMAN GENETIC THERAPIES, INC., Cambridge, MA (US);
Abstract
The invention features a method of producing a high mannose glucocerebrosidase (hmGCB) which includes: providing a cell which is capable of expressing glucocerebrosidase (GCB), and allowing production of GCB having a precursor oligosaccharide under conditions which prevent the removal of at least one mannose residue distal to the pentasaccharide core of the precursor oligosaccharide of GCB, to thereby produce an hmGCB preparation. Preferably, the condition which prevents the removal of at least one mannose residue distal to the pentasaccharide core is inhibition of a class 1 processing mannosidase and/or a class 2 processing mannosidase. The invention also features an hmGCB preparation and methods of using an hmGCB preparation.