The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 31, 2018
Filed:
Dec. 07, 2015
Eastman Chemical Company, Kingsport, TN (US);
North Carolina State University, Raleigh, NC (US);
Stephanie Kay Clendennen, Kingsport, TN (US);
Yaroslava Georgievna Yingling, Cary, NC (US);
Hoshin Kim, Raleigh, NC (US);
Eastman Chemical Company, Kingsport, TN (US);
North Carolina State University, Raleigh, NC (US);
Abstract
The invention relates to amino acid sequence variants of a lipase with improved activity for catalyzing synthesis reactions and methods of preparing the variants. The methods include predicting amino acid sites for change based on computational models of the protein structure in non-aqueous conditions, and expressing the protein in a prokaryotic host for subsequent purification and use. The enzyme sequence variants described have a three to nine-fold improvement in synthesis activity over the parent protein sequence.