Jiangnan University

Jiangsu Guoxin Union Energy Co., Ltd

Zhijie Hu

Cheng Zhang

Xiaodong Jiang

Sai Jin

Fuxin Sun

Jian Chen

Guocheng Du

Jianghua Li

Long Liu

Xian Yin

Jianwei Jiang

Guiyang Shi

Youran Li

Dongjiao Zhou

Jiawei Lu

Maodong Miao

Zihao Fan

Disclosed is anseed continuous culture method, comprising the steps of: (1) at a startup stage,spores are inoculated into a seed culture medium to obtain a seed liquid; (2) at a seed continuous culture stage, continuous dispersion treatment is performed on the seed liquid obtained in step (1), continuous culture is performed on the seed liquid obtained by dispersion, and meanwhile, a fresh seed feed medium is replenished; and (3) at a stop stage, the replenishment of the fresh seed feed medium and the dispersion treatment are stopped, continuous culture is performed to obtain a seed liquid, and then the seed liquid is transferred into the fermentation medium for fermentation culture. The method according to the present invention makes breakthrough to solve problems that multi-cellular filamentous bacteria grow slowly and mycelium pellets are easy to lose in continuous culture, thus fully achieving seed continuous culture.

Aspergillus niger seed continuous culture and method for producing citric acid therefrom

The invention discloses a method for increasing citrate production from genome reconstructed. The method is to insert a gene of low affinity glucose transporter, LGT1, to genome of. The expression level of LGT1 is under control of promoter Pgas. The genome reconstructedis tolerant to higher fermentation temperature and lower pH than that of the parental strain. Moreover, the production, yield and purity of product from reconstructedare higher than that of parental strain, and the fermentation time is shorter.

Method for reconstructing aspergillus niger to increase citrate production

The invention discloses a method for improving citric acid production byfermentation, which integratesGABA pathway succinate semialdehyde dehydrogenase SSD gene intogenome to obtain recombinantstrain, and uses recombinant black Thestrain ferments to produce citric acid; the expression of the succinate semialdehyde dehydrogenase SSD gene is regulated by the Pgas promoter. The method of the invention realizes the expression of succinate semialdehyde dehydrogenase SSD into enhance the GABA pathway so as to strengthen the TCA cycle and promote the synthesis of citric acid.

Method for increasing citric acid production byfermentation

Growing community of inventors

Wuxi, China

Zhijie Hu