Jiangnan University

Zhennan Gu

Hao Zhang

Yongquan Chen

Gang Wang

Haiqin Chen

Jianxin Zhao

Fengwei Tian

Yuanda Song

Wei Chen

Xin Tang

Xiaoyun Huang

Chengfeng Ge

Kai Du

Shanshan Zhao

Tiantian Mei

Haiqun Chen

Guangfei Hao

The present disclosure discloses preparation ofCCFM698 thalli and application thereof in a feed additive, and belongs to the field of biological engineering and feed additives. The total fatty acid content of thedried thalli obtained by the present disclosure is 30%-40% by weight of the dried thalli, and the EPA content is 24% or more by weight of total fatty acids. The dosage of the dried thalli in the feed in the disclosure is 0.5-1.5% of the total weight of the basal feed. The thallus feed additive is reasonable in fatty acid composition and very high in EPA content and can be used for producing high-DHA eggs which are beneficial to body health of eaters. The DHA content in each of eggs laid by laying hens fed with the feed containing the feed additive of the present disclosure reaches about 120 mg which is obviously higher than that in the prior art.

Preparation ofCCFM698 thalli and application thereof in feed additive

It relates to &#x3c9;-3 fatty acid desaturase for the synthesis of pufas from, a plasmid containing the same, a recombinant microorganism containing the plasmid and its application. The invention also provides the use of the above &#x3c9;-3 fatty acid desaturase in the biosynthesis of PUFAs, especially to catalyze C20:4to C20:5at normal temperature, with catalytic efficiency 65%. The recombinant strain reached 31.5% of the total fatty acid, and the conversion rate to AA was up to 77.6%.

Parasitic phytophthora-derived omega-3 fatty acid desaturase for synthesizing polyunsaturated fatty acids, carrier containing fatty acid desaturase, recombinant microorganisms, and application thereof

It relates to aATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention,ATCC32222 is used as a material and undergoes gene knockout through anmediated genetic manipulation technology, to obtain theuracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungusATCC32222 and product development.

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The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially &#x3c9;3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.

Fatty acid desaturase used in the biosynthesis of long chain omega-3 polyunsaturated fatty acids

The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially delta-9 desaturases (FADS9-I). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.

Delta-9 desaturase used in the biosynthesis of polyunsaturated fatty acids

The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially omega-3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.

Omega-3 desaturase used in the biosynthesis of polyunsaturated fatty acids

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Wuxi, China

Zhennan Gu