Other

Massachusetts Institute of Technology

Harvard College

North China Electric Power University

Guodian New Energy Technology Research Institute Co., Ltd.

Tsinghua University

Zhen Xie

Ron Weiss

Zhenyu Chen

Yun Long Jiang

Yinqing Li

He Chen

Yaakov Benenson

Xiangyu Han

Liliana Wroblewska

Zhongwei Lin

Chuanxi Wang

Qingru Cui

Dacheng Ma

Xiongwei Li

Chenzhi Qu

Gengda Li

Weixi Liao

Yun Jiang

The presently claimed invention offers programmable and precise regulation of Cas9 functions by utilizing a set of compact Cas9 derivatives created by deleting conserved HNH and/or REC-C domains based on the structural information across variant class 2 CRISPR effectors. In addition, a novel strategy for engineering the dimeric gRNA-guided nuclease by splitting the mini-dSaCas9 and fusing the FokI domain right after the split point is claimed to increase the on-target DNA cleavage efficiency and potentially reduce the off-target effect because of a closer proximity of dimeric Fold nuclease to the target sequence. By combining the optimized and compact gRNA expression cassette and the downsized SaCas9 derivatives, the entire CRISPR/Cas system with different effector domains for transactivation, DNA cleavage and base editing is loaded into a single AAV virus. Such an all-in-one AAV-CRISPR/Cas9 system will be particularly appealing in biomedical applications that require safe and efficient delivery in vivo.

Engineering of a minimal SaCas9 CRISPR/Cas system for gene editing and transcriptional regulation optimized by enhanced guide RNA

The disclosure proposes a yaw calibration method and system for a wind turbine. The calibration method includes: establishing a cylindrical coordinate graph of wind resource distribution based on historical wind farm operation data, to determine a wind direction interval of main inflow wind conditions of a wind farm; calculating an effective value of active power of each wind speed sub-interval, and obtaining a fitted power curve of each refined interval through curve fitting; and setting an angle between a central axis of a refined interval for a fitted power curve corresponding to a calibration curve in each wind speed range and a central axis of the to-be-calibrated wind direction interval as a yaw error calibration value in the wind speed range, to establish a wind speed-yaw error calibration value lookup table; and determining a yaw error calibration value under a current wind direction and a current wind.

Yaw calibration method and system for wind turbine

Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors. Provided is a method for achieving regulated expression of two proteins: an expression cassette A comprises a feedback element coding sequence, a promoter A, a protein A and TALER protein A encoding gene linked by means of a self-cleaving polypeptide, and a target sequence A (comprising an shRNA1 target sequence); an expression cassette B comprises a feedback element coding sequence, a promoter B, a protein B and TALER protein B encoding gene linked by means of a self-cleaving polypeptide, and a target sequence B (comprising an shRNA2 target sequence); an expression cassette C comprises a constitutive promoter and an activating element-coding sequence; a recombinant vector A having the expression cassette A, a recombinant vector B having the expression cassette B, and a recombinant vector C having the expression cassette C are introduced into host cells to regulate the expression of protein A and protein B by adding shRNA1 or shRNA2.

Modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors

Provided herein are high-input detector modules and multi-input biological classifier circuits and systems that integrate sophisticated sensing, information processing, and actuation in living cells and permit new directions in basic biology, biotechnology and medicine. The multi-input biological classifier circuits described herein comprise synthetic, scaleable transcriptional/post-transcriptional regulatory circuits that are designed to interrogate the status of a cell by simultaneously sensing expression levels of multiple endogenous inputs, such as microRNAs. The classifier circuits then compute whether to trigger a desired output or response if the expression levels match a pre-determined profile of interest.

Multiple input biologic classifier circuits for cells

Growing community of inventors

Beijing, China

Zhen Xie