Fuso Pharmaceutical Industries, Ltd.

Hokkaido University

Nextier Corporation

Yuzuru Kitahara

Keiichi Yamamoto

Yasuhiko Suzuki

Yusuke Suzuki

Hiroshi Tahara

Toru Shinzato

Satoru Konnai

Kazuhiko Ohashi

Shiro Murata

Tomohiro Okagawa

Naoya Maekawa

Yasuyo Maruyama

Miki Nakagawa

Yayoi Kameda

Shinya Goto

Yamato Sajiki

The present invention provides mammalian cell expression vectors that impart to mammalian host cells an ability to produce high levels of foreign gene-derived proteins. A ubiquitously acting chromatin opening element (UCOE) is introduced into an expression vector that has a plasmid DNA integrated into a transcriptional hot spot on the chromosome of a dihydrofolate reductase gene-deficient host cell so that it allows for selection of strains that grow in hypoxanthine-thymidine (hereinafter denoted as HT)-free medium, whereby transformants will produce a protein of interest in increased amounts.

Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element

The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.

Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof

A respiratory tract widening tool capable of reliably managing a respiratory tract in a supine posture and freely changing the posture, and a respiratory tract widening unit provided therewith are provided.

Respiratory tract widening tool and respiratory tract widening unit provided therewith

As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.

Protein substance having triple helix structure and manufacturing method therefor

Growing community of inventors

Osaka, Japan

Yuzuru Kitahara