Japan Tobacco Inc.

Kaneka Corporation

Chinese Academy of Agricultural Sciences

Yuji Ishida

Yukoh Hiei

Yoshimitsu Takakura

Toshihiko Komari

Keisuke Kasaoka

Satoru Usami

Shigeru Kuwata

Yasuhiro Inoue

Teruyuki Imayama

Fumiki Tsutsumi

Jing Wang

Takeshi Yamamoto

Ke Fei Wang

Lei Shi

Jun Ueki

Toshiki Mine

Toshiyuki Komori

Shozo Ohta

Hideaki Saito

Huiyun Liu

Tomoaki Kubo

Xingguo Ye

Chizu Yanagihara

Kunyang Wang

Lipu Du

Zhishan Lin

The present invention provides a method for improving transformation efficiency of a plant. The method according to the present invention comprises the use of a nucleic acid encoding the amino acid sequence set forth in SEQ ID NO: 2 or 4, or a nucleic acid encoding a polypeptide comprising an amino acid sequence having at least 85% identity with the amino acid sequence set forth in SEQ ID NO: 2 or 4, and having a function of improving transformation efficiency of a plant.

Method for improving transformation efficiency of plant and method for transforming plant

This invention relates to thebacterium to be used in plant transformation method comprising three types of plasmids. Thebacterium of this invention comprises plasmids of (1) to (3) shown below:

Agrobacterium bacterium to be used in plant transformation method

A method for gene introduction by which higher efficiency for gene introduction than that by the conventionalmethod may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene introduction into plant cells by a bacterium belonging to genusis promoted by accompanying centrifugation of the plant cells or plant tissue.

Method for promoting efficiency of gene introduction into plant cells

An object of the present invention is to provide a method of gene introduction, which can transform aplant at a higher efficiency compared to that in knownmethods, and a method of producing a transformedplant. The method of the invention includes a step of subjecting an immature embryo tissue of aplant to centrifugation treatment and/or pressurization treatment before the inoculation with, during the coculture step, and/or after the coculture step, and is characterized in that the coculture medium satisfies at least one of a) containing an antiauxin; b) containing a cytokinin; and c) containing a phenoxy auxin in an amount of less than 2 &#x3bc;M and/or a benzoic auxin in an amount of less than 5 &#x3bc;M, or not containing any phenoxy auxin and/or benzoic auxin.

Method for gene introduction into hordeum plant using agrobacterium, and method for production of transformed plant of hordeum plant

The method of the present invention includes the step of excising one or more portions selected from a radicle, a germ, and an embryonic axis of a plant tissue inoculated withafter cultivation in a coculture medium. The present invention provides a method of gene introduction that can transform aplant at high efficiency compared to conventionally knownmethods, and provides a method of producing a transformed plant.

[object Object]

An-mediated method for producing transformed maize or rice, culturing an-inoculated immature embryo with a coculture medium and a regeneration step for culturing the immature embryo with a regeneration medium either without callus growth or after callus growth culture to regenerate whole transformed maize or rice. The method further includes a transformation enhancement and the method does not include any selection step based on the properties of a nucleic acid to be introduced byin any step from coculture to regeneration.

Agrobacterium-mediated method for producing transformed maize or rice

The present invention provides a method for-mediated gene transfer into a plant material, which comprises inoculating aninto the plant material in the presence of a powder. In the method of the present invention, the powder at least does not affect living tissues and has one or more properties selected from the group consisting of: being insoluble in water; having an affinity for living tissues; having adsorption properties; and having a surface polarity. The present invention also provides a method for producing a transformed plant, which comprises using the gene transfer method of the present invention.

Method for improving transformation efficiency using powder

The present invention aims to provide novel vectors for plant transformation. The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that:

Cosmid vector for plant transformation and use thereof

The present invention aims to provide a method for increasing transformation efficiency in plants when compared to conventionally known-mediated methods. In the present invention, one of the features is to comprise a coculture step for culturing an-inoculated plant tissue with a coculture medium containing 3,6-dichloro-o-anisic acid.

Method for increasing transformation efficiency in plants, comprising coculture step for culturing plant tissue with coculture medium containing 3,6-dichloro-o-anisic acid

A novel-mediated method for producing a transformed monocotyledonous plant is provided. The transformation method involves (i) a coculture step for culturing an-inoculated monocotyledonous plant tissue with a coculture medium containing 3,6-dichloro-o-anisic acid, 4-amino-3,5,6-trichloropicolinic acid and/or 2,4,5-trichlorophenoxyacetic acid, and (ii) a regeneration step for culturing the tissue obtained in (i) with a regeneration medium containing a selective drug to thereby induce regeneration and to produce a transformed plant. The method does not include, between the coculture step and the regeneration step, any selection step for culturing the cocultured tissue with a medium containing an auxin and a selective drug to select a transformant.

Agrobacterium-mediated method for producing transformed plant without selection step

The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement. The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.

Method for screening genomic DNA fragments

Disclosed is a method of transforming monocotyledons which necessitates only a short period from the transformation to the regeneration of a whole plant as compared with the conventional methods, thus reducing the frequency of occurrence of mutants, and can be generally applied to the plants for which any system of regenerating the whole plants from protoplasts has not been established, and in which the material to be used can be readily prepared without any particular apparatuses. The present invention provides a method for transforming monocotyledons comprising transforming scutellum of an immature embryo of a monocotyledon with a bacterium belonging to genuscontaining a desired gene, which immature embryo has not been subjected to a dedifferentiation treatment, to obtain a transformant.

Method of transforming monocotyledons using scutella of immature embryos

A method for gene transfer by which higher efficiency for gene transfer than that by the conventionalmethod may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene transfer into plant cells by a bacterium belonging to genusis promoted by accompanying heat treatment and centrifugation treatment of the plant cells or plant tissue.

Method of improving gene transfer efficiency into plant cells utilizing heat and centrifugation

The present invention provides transgenic, disease-resistant plants which have been transformed with an expression cassette including a constitutive gene expression promoter; and a gene, under the control of the promoter, encoding a harpin. The transformed cells in the transgenic plant effect the constitutive expression of the harpin in an amount effective for inducing a defense reaction. The harpin is a protein consisting of an amino acid sequence that is at least 90% homologous to the amino acid sequence of SEQ ID NO: 2, and possesses a hypersensitive-response-inducing activity. The present invention also provides methods for producing the transgenic plants, expression cassettes, recombinant vectors and genes encoding harpins.

Growing community of inventors

Iwata, Japan

Yuji Ishida