Johns Hopkins University

Human Genome Sciences, Inc.

Takara Shuzo Co., Inc.

Other

University of Wyoming

Artchem, Inc.

Yuan C Lee

Timothy A Coleman

Shawn M Lawrence

Michael J Betenbaugh

Ikunoshin Kato

Jon J Hangeland

Donald L Jarvis

Paul O Ts'o

Kazuaki Kakehi

Akihiro Kondo

Shojiro Iwahara

Kaoru Takegawa

Susumu Honda

Akimasa Miwa

Akinori Tsujimoto

Shinji Inoue

Jian Qiang Fan

Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.

Engineering intracellular sialylation pathways

Oligodeoxynucleoside methylphosphonate neoglycopeptide conjugates and related compounds for tissue specific delivery of biologically stable, nonionic oligodeoxynucleoside analogs into cells.

Ligands to enhance cellular uptake of biomolecules

To provide a process for producing a carbohydrate or a glycoconjugate without forming any hydrolytic product of the remodeled sugar chain. A process for producing a carbohydrate or a glycoconjugate by a remodeling reaction in the presence of a glycosidase which performs the remodeling reaction in an aqueous medium containing a water-soluble ketone and/or dioxane. Examples of the glycosidase include endoglycosidases such as Endo-A. Examples of the ketone include acetone.

Process for producing carbohydrate or glycoconjugate

A connection type fluid transfer and treatment system apparatus and method for efficiently and continuously executing transfer and treatment of small or micro amounts of sample solutions without substantial transfer loss, which includes a first microsolution reaction microtube having one open end and a second closed end, a second microsolution target microtube having substantially the same shape as the first microtube also having one open end and one closed end, and a connector for connecting together the open ends of the first microtube to the open end of the second tube. The connector includes a foramenous membrane support, which removably receives chemically or biologically treated membranes for applying a predetermined treatment to a solution while passing the sample solution from the first microtube to the second tube. Alternately, a 'single use only' connector having disposed therein a membrane pretreated with a quantitative amount of reagent may be used for certain treatment operations. The single use only connector is used once and then discarded. An appropriate color indicator in the connector or membrane would serve to indicate whether the connector had been used. The sample is typically filtered through the membrane by centrifugation. The assembly also includes special adapters for receivingly engaging the dual tube/connector transfer system during centrifugation. The system permits handling microliter quantities of reactive solutions in biochemical analyses, treatments and assays without use of micropipets, without the usual loss of solution. An enzyme microsolution test kit system and method of use comprising one or more micro solution microtubes containing a predetermined quantity of a known, preferably lyophilized, reagent pre-coated on the microtube walls is disclosed.

Connection-type treatment system for micro solution and method of

Disclosed herein is a method of labelling a sugar at its reducing end by using 1-(p-methoxyphenyl)-3-methyl-5-pyrazolone, thereby analyzing said sugar with high sensitivity, and to a kit to be used for sugar labelling by this method.

Method for labelling sugars

Growing community of inventors

Timonium, MD, United States of America

Yuan C Lee