Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Bio Co., Ltd.

Other

Yoshiyuki Yonetani

Shin-ichi Hashimoto

Akio Ozaki

Mikiro Hayashi

Kazuhiko Tabata

Hajime Ikeda

Makoto Yagasaki

Hiroshi Mizoguchi

Hirofumi Endo

Keiko Ochiai

Kenji Soda

Masaki Maeda

Haruhiko Yokoi

Takeshi Takahashi

Masato Satomura

Hiroshi Kawamoto

Tadashi Inaba

Masanobu Takashima

Hiroshi Takeuchi

Satoshi Koizumi

Yoshihisa Tsukada

Hiroshi Kitaguchi

Tamio Mizukami

Tatsuya Tamaoki

Naoki Asanuma

Sadao Teshiba

Shun-ichi Ikeda

Junko Ohnishi

Seiko Ando

Akihiro Senoo

Yuko Kanda

Yugo Adachi

Shin-Ichi Hashimoto

Provided is an efficient process for producing L-glutamine or L-glutamic acid using a microorganism. L-glutamine or L-glutamic acid is produced by culturing in a medium a microorganism in which has an ability to produce L-glutamine or L-glutamic acid, and in which an ability to form a superhelical double-stranded DNA is decreased compared with that of the parent strain, producing and accumulating L-glutamine or L-glutamic acid in the medium, and recovering L-glutamine or L-glutamic acid from the medium.

Process for production of L-glutamine or L-glutamic acid

The invention provides a microorganism having an ability to produce a protein having a dipeptide synthesizing activity and in which an activity of the protein to transport a dipeptide in a microbial cell to theoutside of the microbial cell is higher than that of a parental strain.

Method for producing dipeptide

The invention provides a process for producing glutathione or &#x3b3;-glutamylcysteine by culturing in a medium a microorganism with a higher activity of a protein having an activity to transport intracellular glutathione to the outside of cells, and a higher activity of a protein involved in glutathione or &#x3b3;-glutamylcysteine biosynthesis, compared with that of the parent strain, forming and accumulating glutathione or &#x3b3;-glutamylcysteine in the medium, and recovering the glutathione or &#x3b3;-glutamylcysteine from the culture.

Method for production of glutathione or gamma-glutamylcysteine

According to the present invention, it is possible to provide a microorganism belonging to the genus, wherein the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, or (2) a protein having a homology of 80% or more to the amino acid sequence shown by any one of SEQ ID NO:1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, has been reduced or lost, and wherein the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO:4, or (4) a protein having a homology of 80% or more to the amino acid sequence shown by SEQ ID NO:4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO:4, has been reduced or lost, and a process for producing L-glutamine using the microorganism and the like.

Method for production of L-glutamine

A polypeptide comprising an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence of a glutamine synthetase 2 derived from a microorganism belonging to a coryneform bacterium, wherein the coryneform bacterium producing the polypeptide has L-glutamine productivity, a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism comprising the DNA or the recombinant DNA, and a process for producing L-glutamine using the microorganism are provided.

The present invention provides a protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for proteins of the amino acid sequences as shown in SEQ ID NOS: 4 to 6), and a process for industrially advantageously producing a compound that inhibits HMG-CoA reductase and has an action to decrease serum cholesterol, using DNA encoding the protein comprising the amino acid sequence as shown in any one of SEQ ID NOS: 1 to 3 (except for DNA encoding the protein comprising the amino acid sequence as shown in SEQ ID NO: 4).

Protein and DNA encoding the protein

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein Rrepresents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and Rrepresents a substituted or unsubstituted alkyl or a substituted or unsubstituted aryl,

Process for producing HMG-CoA reductase inhibitor

The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.

Low-substrate-specific amino acid racemase and process for producing racemic amino acid

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): 

DNA encoding hydroxylase

Amino acid racemase having low substrate specificity and process for producing racemic amino acid

The present invention relates to: a process for producing D-serine wherein a microbial cell which is modified to have a higher L-serine deaminase activity thanDH5&#x3b1; strain, a culture of said cell, or a processed product thereof is brought into contact with DL-serine in a DL-serine-containing medium to decompose L-serine, and the remaining D-serine is recovered from the medium; and a microorganism used for this production process. D-serine is a useful compound as a synthetic intermediate for useful medicaments such as D-cycloserine.

Process for producing D-serine

The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as 'G6PD') derived from a bacterium belonging to the genus, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant. According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.

Glucose-6-phosphate dehydrogenase

The present invention relates to a protein derived from a microorganism belonging to the genus, which has an activity of hydroxylating a compound represented by the formula (I-a): wherein Rrepresents a hydrogen atom, a substituted or unsubstituted alkyl, or an alkali metal, and Rrepresents a substituted or unsubstituted alkyl or a substituted or unsubstituted aryl,or a ring-closed lactone form thereof;a DNA encoding the protein; and a recombinant DNA comprising the DNA.

The present invention relates to a process for producing a compound (II-a) or a compound (II-b), each of which is a hydroxylated product of a compound represented by the formula (I-a) (hereinafter referred to as compound (I-a)):wherein

Process for producing HMG-CoA reductase inhibitors

The present invention relates to a novel aspartokinase derived from a Coryneform bacterium; a DNA encoding the enzyme; a recombinant DNA containing the above DNA; a Coryneform bacterium having the above recombinant DNA or a Coryneform bacterium having the DNA on its chromosome; and a process for producing L-lysine by culturing the above microorganism. Construction has been successfully made of a DNA encoding an aspartokinase freed from concerted feedback inhibition by L-lysine and L-threonine derived from aand has a nucleotide sequence encoding an amino acid sequence wherein the amino acid residue at position 311 is an amino acid other than Thr in the amino acid sequence shown by SEQ ID NO: 18.

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Machida, Japan

Yoshiyuki Yonetani