Ajinomoto Co., Ltd.

Yoko Asakura

Hisao Ito

Mikiko Suga

Osamu Kurahashi

Tsuyoshi Nakamatsu

Eiichiro Kimura

Masakazu Sugimoto

Kazuhiko Matsui

Yoshio Kawahara

Jun Nakamura

Yukiko Mori

Tsuyoshi Ohsumi

Sohei Kanno

Yoshihiro Usuda

Hisashi Yasueda

Takuji Ueda

Yasuhiko Yoshihara

Nobuharu Tsujimoto

Yuri Nagai

Akinori Uehara

Ippei Inoue

Sumio Inoue

Chizu Abe

A method for producing an L-amino acid is described, which is characterized by culturing abacterium capable of producing the L-amino acid in a culture medium to produce and accumulate the L-amino acid in the culture medium and collecting the L-amino acid from the culture medium.

Method for production of L-amino acid

An L-amino acid is produced by culturing a microorganism which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, wherein the bacterium has been modified to enhance orotate phosphoribosyltransferase activity is enhanced, in a medium to produce and cause accumulation of an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

L-amino acid-producing microorganism and a method for producing an L-amino acid

A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.

Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains

L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.

Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine

The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained fromchromosomal DNA library by cloning a DNA fragment that complicates serB deficiency ofas a open reading frame in the DNA fragment.

Phosphoserine phosphatase of coryneform bacteria and variants thereof

The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained fromchromosomal DNA library by cloning a DNA fragment that complicates serB deficiency ofas a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.

Phosphoserine phosphatase gene of coryneform bacteria

A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.

.alpha.-Ketoglutarate dehydrogenase gene

Methods of producing L-lysine and L-glutamic acid by fermentation

Growing community of inventors

Kawasaki, Japan

Yoko Asakura