National Institute of Advanced Industrial Science and Technology

Kankyo Engineering Co., Ltd.

Other

Japan Bioindustry Association

Agency of Industrial Science and Technology

Japan As Represented by Secretary of Agency of Industrial Science and Technology

Yoichi Kamagata

Ryuichiro Kurane

Shinya Kurata

Takahiro Kanagawa

Kazutaka Yamada

Toyokazu Yokomaku

Osamu Koyama

Kenta Furusho

Masaki Torimura

Satoru Ohgiya

Takehiko Sahara

Kazuhiro Fujimori

Yosuke Kobayashi

Takashi Akamatsu

Taisuke Seike

Hisataka Taguchi

The present invention provides information on a mutant xylose isomerase gene and a mutant protein with which it is possible to impart high xylose metabolic capacity to budding yeast. Also provided is a yeast strain having the mutant xylose isomerase gene. Additionally provided is an efficient method for producing a useful substance using the yeast strain. The present invention provides mutant--derived xylose isomerase (CpXI) having high xylose metabolic activity, the CpXI comprising an amino acid sequence corresponding to an amino acid sequence in which the number 63 threonine of SEQ ID NO: 11 of CpXI is substituted by isoleucine, leucine, glycine, or histidine and/or the number 162 valine is substituted by alanine. Also provided are: a transformed yeast having high ethanol productivity, the transformed yeast being obtained by transformation using a mutant CpXI gene that comprises a codon mutation corresponding to a codon mutation in which the number 63 threonine of SEQ ID NO: 11 in a CpXI gene optimized for the preferred codon of budding yeast is substituted by isoleucine, leucine, glycine, or histidine and/or the number 162 valine is substituted by alanine; and a method for producing ethanol using the transformed yeast.

Mutant xylose-metabolizing enzyme and use thereof

The present invention pertains to: mutant genes having a mutation such as a base substitution in the MTH1, GRR1 and/or CDC19 coding regions thereof; mutant proteins coded by said mutant genes; an upstream region of the GRR1 coding region having a mutation such as a base substitution; a yeast such ashaving said upstream region; and a method for producing a substance such as ethanol by using said yeast.

Yeast having enhanced xylose fermentation ability, and use therefor

A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.

Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method

A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises:

Methods for monitoring PCR amplification with fluorescently labeled probes or primers for which fluorescence changes upon hybridization to a target

Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.

Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method

A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.

Nucleic acid probe and novel method of assaying nucleic acid using the same

Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method

Growing community of inventors

Tukuba, Japan

Yoichi Kamagata