International Reagents Corporation

Sysmex Corporation

Amano Pharmaceutical Co., Ltd.

Yasushi Shirahase

Yoshifumi Watazu

Tadahiro Kajita

Kazuaki Yamashita

Koji Kishi

Shinichi Sakasegawa

Tsutomu Kakuyama

Kenji Konishi

Toshiyuki Yoshida

Tadashi Hoshino

Kenji Isshiki

Osamu Hotta

Disclosed are methods and reagents for the enzymatic measurement of short-chain fatty acids, having 3 to 6 carbon atoms, in a sample. Methods include the use of recombinant butyrate kinases from multiple species, combined various reagents that includes ATP, to detect butyric acid in different types of samples via measurement of ATP consumption. Disclosed also are the reagents themselves.

Enzymes and reagents for measurement of short chain fatty acids

The object of the present invention is to provide a method for more accurately assaying the enzyme activities of mCK isozymes and a method for separately assaying the enzyme activities of CK isozymes by separately assaying the enzyme activities of ubiquitous mCK (umCK) and sarcomeric mCK (smCK). The above object is attained by an assay using an antibody that specifically recognizes umCK protein. In addition, other anti-mCK antibodies (e.g., anti-smCK antibody) and/or anti-human CK-M-inhibiting antibody can also be used. The above antibody is a polyclonal antibody or a monoclonal antibody. As a result of these antibodies, a monoclonal antibody (U1-1881) that is capable of specifically recognizing human umCK and is produced by a hybridoma having a deposition number of FERM BP-8342 is provided.

Mitochondrial creatine kinase antibody

Effective stabilizing amount at least of one antioxidant is added to a composition containing an esterase and surfactant(s).

Reagent for assaying lipid

A method for measuring the activity of creatine kinase MB (CK-MB) isozyme which is accurate, high in specificity, convenient, by inhibiting the activity of a mitochondria localized creatine kinase (mCK) isozyme to avoid the influence of mCK and a measurement reagent therefor.

Method for assaying creatine kinase isozyme activity and assay reagent

By a process comprising inhibiting LDH,, LDH,, LDH,and LDH,in a sample with a protease in the presence of a protein-denaturating agent and then determining LDH,remaining uninhibited, LDH,in the sample is easily determined.

LDH1 assay

A method for assaying biological specimens for substances contained in the components of each specimen by the use of one or more kinds of calixarenes; and a reagent comprising one or more kinds of calixarenes. The method utilizes complexes formed by calixarenes and the components of biological specimens, and makes it possible to assay biological specimens for substances contained in the components of each specimen, for example, for cholesterol contained in high-density lipoprotein (HDL), without preliminary separation from the other components of the biological specimen. The method can be conducted by easy and simple operations and lessen measurement errors or problems caused by man, and permits continuous measurement with general-purpose automatic analyzing apparatuses and multi-channel measurement together with other test items.

Method for assaying biological specimens for substances contained in the

A glutamic-oxaloacetic transaminase (GOT) isozyme assay, which comprises selectively inhibiting s-GOT in a sample such as serum or plasma by proteinase K, optionally an excess amount of proteinase K by a protease inhibitor and then determining m-GOT remaining intact, is described.

Growing community of inventors

Kobe, Japan

Yasushi Shirahase