Hitachi-high-technologies Corporation

Shionogi Company Limited

Yasuhiro Takegawa

Shinya Ito

Kisaburo Deguchi

Shin-Ichiro Nishimura

Izumi Ogata

Shinichiro Nishimura

It is intended to chemically synthesize the trisaccharide moiety at the reducing end in the core sugar chain structure of an asparagine-linked glycoprotein. By using a highly inexpensive natural polysaccharide having a mannose&#x3b2;-1,4-bond as the starting material, a &#x3b2;-1,4-glycoside bond of mannose is formed.

Synthesis of core sugar chain structure of asparagine-linked glycoprotein

The invention intends to determine a presence ratio between mixed compounds which are difficult to separate from each other by a separation unit and cannot be discriminated by MS. In a mass spectrometer with an MSanalysis function for ionizing a sample eluted from a separation unit for separating the sample into individual components, fragmenting an ion of a desired mass number, and performing a mass analysis of fragment ions, the mass spectrometer includes a database storing correlation information between an isomer presence ratio and a particular ion intensity ratio in a mass spectrum per isomer. An isomer ratio can be clarified even for a mixed sample of isomers, such as enantiomers, which are difficult to separate from each other by the separation unit and cannot be discriminated by MS.

Mass spectrometer and method of analyzing isomers

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Sapporo, Japan

Yasuhiro Takegawa