University of California

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Eastman Chemical Company

North Carolina State University

Yaroslava Georgievna Yingling

Stephanie Kay Clendennen

Kirill Andreevich Afonin

Bruce Allen Shapiro

Luc Jaeger

Wojciech Kasprzak

Eckart Bindewald

Hoshin Kim

Isil Severcan

Cody Geary

The invention relates to amino acid sequence variants of a lipase with improved activity for catalyzing synthesis reactions and methods of preparing the variants. The methods include predicting amino acid sites for change based on computational models of the protein structure in non-aqueous conditions, and expressing the protein in a prokaryotic host for subsequent purification and use. The enzyme sequence variants described have a three to nine-fold improvement in synthesis activity over the parent protein sequence.

CALB variants

The instant invention provides polyvalent RNA nanoparticles comprising RNA motifs as building blocks that can form RNA nanotubes. The polyvalent RNA nanoparticles are suitable for therapeutic or diagnostic use in a number of diseases or disorders.

RNA nanoparticles and nanotubes

Growing community of inventors

Cary, NC, United States of America

Yaroslava Georgievna Yingling