Research Development Foundation

Xue-Jie Yu

David H Walker

Jere W McBride

The 28-kDa outer membrane proteins (P28) ofare encoded by a multigene family consisting of 21 members located in a 23-kb DNA fragment in the genome of. Fifteen of these proteins are claimed herein as novel sequences. The amino acid sequence identity of the various P28 proteins was 20-83%. Six of 10 tested p28 genes were actively transcribed in cell culture grown. RT-PCR also indicated that each of the p28 genes was monocistronic. These results suggest that the p28 genes are active genes and encode polymorphic forms of the P28 proteins. The P28s were also divergent among different isolates of. The large repertoire of the p28 genes in a single ehrlichial organism and antigenic diversity of the P28 among the isolates ofsuggest that the P28s may be involved in immune avoidance.

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The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of. Recombinant28-kDa proteins react with convalescent phase antiserum from an-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.

The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family of . Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes of . Recombinant 28-kDa proteins react with convalescent phase antiserum from an -infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.

Homologous 28-kilodalton immunodominant protein genes of Ehrlichia canis and uses thereof

The present invention is directed to the cloning, sequencing and expression of a conserved immunoreactive 28-kDa protein gene (P28) from a polymorphic multiple gene family of P28 has an 834-bp open reading frame encoding a protein of 278 amino acids with four variable regions, and shares similar surface-exposed regions, antigenicity and T-cell motifs with P28. Also disclosed is that recombinant P28 protein reacts with convalescent phase antiserum from an -infected dog.

28-kDa immunoreactive protein gene of Ehrlichia canis and uses thereof

The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, ECa28-1 and ECa28SA3, from a polymorphic multiple gene family of A complete sequence of another 28-kDa protein gene, ECaSA2, is also provided. Further disclosed is a multigene locus encoding all five homologous 28-kDa protein genes of Recombinant 28-kDa proteins react with convalescent phase antiserum from an -infected dog.

Homologous 28-kilodalton immunodominant protein genes of ehrlichia canis and uses thereof

Homologous 28-kilodalton immunodominant protein genes of Ehrlicha canis and uses thereof

The present invention provides a 120-kDa protein gene of Ehrlichia canis, amplified by PCR using primers derived from the DNA sequences flanking the Ehrlichia chaffeensis 120-kDa protein gene. The recombinant E. canis 120-kDa protein contains 14 tandem repeat units with 36 amino acids each. The repeat units are hydrophilic and predicted to be surface-exposed. Also disclosed is that the recombinant E. canis 120-kDa protein is antigenic and reacts with sera from dogs convalescent from canine ehrlichiosis.

Ehrlichia canis 120-kDa immunodominant antigenic protein and gene

Disclosed is an isolated gene encoding a 120 kDa immunodominant antigen of Ehrlichia chaffeensis. The 120-kDa protein is one of the immunodominant proteins of E. chaffeensis that stimulates production of specific antibodies in infected humans. Also disclosed are the amino acid sequence of the 120 kDa antigen. Methods of producing a recombinant 120 kDa antigen and therapeutic methods of use of the antigen are also disclosed.

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Galveston, TX, United States of America

Xue-Jie Yu