Other

Advanced Research & Technology Institute

Xu-Shan Wang

Arun Srivastava

Selvarangan Ponnazhagan

Jaime A Escobedo

Mervin C Yoder

Varavani J Dwarki

Shang-Zhen Zhou

Keyun Qing

Robert H Chloemer

Anil Bajpai

The present invention identifies a protein, designated the D-sequence-binding protein (D-BP), is phosphorylated at tyrosine residues and blocks AAV-mediated transgene expression in infected cells by inhibiting the leading strand viral DNA synthesis. More particularly, the present invention demonstrates that D-BP is phosphorylated by EGF-R protein tyrosine kinase. Methods of increasing transcription and promoting replication of transgenes exploiting this information are disclosed herein.

Role of tyrosine phosphorylation of a cellular protein in adeno-associated virus 2-mediated transgene expression

The present invention is directed to a recombinant adenovirus vector comprising two inverted terminal repeats (ITRs) each of which comprises a D-sequence having (i) from 5 to 15 native nucleotides and (ii) one or more deletions or substitutions therein.

AAV vectors

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Carmel, IN, United States of America

Xu-Shan Wang