Nanjing Hanxin Pharmaceutical Technology Co., Ltd.

Amphastar Nanjing Pharmaceuticals Inc.

Xiaoyang Fan

Haoning Zhang

Song Chen

Chuangen Tang

Jing Wang

Shangshu Pan

Xiaorui Liu

Bo Jin

Teng Zhang

The invention discloses a fusion protein and its preparation method for intermediate polypeptide of Semaglutide. The invention belongs to the technical field of genetic engineering and polypeptide preparation. The fusion protein comprises a fusion peptide, a protease cleavage site and a target main molecular sequence. By optimizing the fusion peptide sequence, changing the isoelectric point and hydrophilicity of the protein, the highest expression of the fusion protein is effectively increased to 13.1 g/L. Meanwhile, the properties of fusion protein are also improved, which is conducive to the development of subsequent extraction, enzyme digestion and purification processes. The yield of intermediate polypeptide after enzyme digestion is 3.62 g/L. The production cost of Semaglutide intermediate polypeptide Arg34GLP-1(9-37) is reduced from the source, which is conducive to industrial scale-up and suitable for industrial production.

Fusion protein and its preparation method for intermediate polypeptide of Semaglutide

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Nanjing, China

Xiaoyang Fan