Jiangnan University

Xiaoling Guo

Min Zhang

Lihua Hou

Sifu Yi

Wuxiong Yang

Hao Shi

Yanyan Lao

The present application belongs to the technical field of research and development of fermented beverages, and a method for preparingferment by two-stage fermentation and complex enzymatic hydrolysis is provided. The present application involves preparation of apowder and a Cordyceps militaris slurry, preparation of afermentation substrate, lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and yeast fermentation, followed by centrifugation, filtration, formulation, sterilization, and filling to obtain a product. By employing lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and secondary fermentation by yeast, the present application can quickly finish a whole fermentation process in 2 to 3 days.

Method for preparing Cordyceps militaris ferment by two-stage fermentation and complex enzymatic hydrolysis

Disclosed is an ultrasonic composite acidic water extraction method for apolysaccharide and cordycepin in, which falls within the technical field of food processing. The method comprises: picking, washing, drying, pulverizing and degreasingfruiting bodies to obtain adry powder; then immersing same in a prepared diluted hydrochloric acid solution to perform three cycles of ultra-low temperature freezing and microwave defrosting, and at the same time using low-frequency ultrasonic waves to carry out assisted stirring and extraction; then subjecting an extract to evaporation and concentration treatments; and finally, performing freeze-drying on the extract to collect a dry powder of a water extraction product. The amount of an additive used in the method meets domestic and foreign usage requirements of food additives, and the operation is simple, practical and uses a combination of chemical and physical methods, thereby saving time and being highly efficient.

Ultrasonic composite acidic water extraction method for cordyceps polysaccharide and cordycepin in

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