Miami University

Xiao-Wen Cheng

Tyler A Garretson

Xin-Hua Cheng

Hui Shang

Certain donor plasmid vectors such as pFastBac&#x2122;1 and pFastBac&#x2122; Dual lack a cis DNA element upstream of the polh translation start codon (ATG) present in wild type (wt)multiple nucleopolyhedrovirus (AcMNPV), and contain a SV40 pA fragment. When a cis DNA element is inserted upstream of the 50 bp polh promoter and SV40 pA was replaced with a AcMNPV polh pA signal in pFastBac&#x2122;1 and pFastBac&#x2122;Dual, certain protein expression levels in High Five&#x2122; cells using the Bac-to-Bac&#xae; system reached that of the wt AcMNPV.

Donor plasmid vectors

A recombinant baculovirus expression vector or cell comprising an engineered baculovirus fp25k gene with one to three modified or mutated spots, the modified spots comprise the two 7-adenine mononucleotide repeats (MNR) and the 10TTAA site. The invention also provides the method of making the vector and baculovirus.

Growing community of inventors

Oxford, OH, United States of America

Xiao-Wen Cheng