Anhui Agricultural University

Institute of Protection and Agro-Products Safety, Anhui Academy of Agricultural Science

Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences

Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences

Xianyan Su

Yu Chi

Zhenghe Ye

Lina Xu

Xuexiang Ren

Yong Zhang

Tingting Xu

Chengqi Zhang

Sijia Bi

Youmin Tong

Xiujun Wu

Li Chen

Feilong Wu

Haoliang Chen

Fei Hu

Mengqiu Wang

Benjin Hu

The present invention relates to the field of microbial culture, in particular to a method for expanding propagation and producing spores ofand use thereof. In the present invention,Mr006 is sprayed on 2nd instar live larvae oftreated with a sub-lethal dose of chlorantraniliprole to cause infection, the 2nd instar larvae of theinfected with theMr006 spores are concentrated into a sterile box, placed into an artificial climate box and continuously fed with an artificial feed until death or pupation, the infection condition of the insect bodies is observed, spores are collected after a 1st complete spore production, after the infected insects are subjected to moisture preservation, expanded propagation and culture are continuously performed, and the spores are repeatedly collected twice to obtain the maximum spore yield.

Method for expanding propagation ofMR006 and use thereof

The present disclosure provides a pyraclostrobin microcapsule based on sodium alginate, which is prepared by mixing pyraclostrobin original drug dissolved in ethyl acetate, 1% aqueous sodium alginate solution, and 5% aqueous calcium carbonate solution at 25° C., and mixing the mixed solution with liquid paraffin containing 10% emulsifier A-110, maintaining a shear rate of 1000 r/min to emulsify for 10 min; using 87.5 mmol/L glacial acetic acid to reduce the pH of the mixed phase, such that the cross-linking reaction between ions can be formed into microcapsules; finally using pH 4.5 acetate buffer to wash and separate the microcapsule slurry, and then centrifuging and freeze-drying to prepare the pyraclostrobin SA microcapsules. The microcapsules prepared by the present disclosure have excellent formulation performance and can be used for the control of Fusarium pseudograminearum.

Pyraclostrobin microcapsule based on sodium alginate and preparation method

Disclosed is a pesticide composition for controlling. The pesticide includes an auxiliary material, an active ingredient A and an active ingredient B. The active ingredient A is tea saponin, the active ingredient B is chlorantraniliprole or tolfenpyrad, and the mass ratio of the active ingredient A to the active ingredient B is 1-20:20-1. In the present invention, by mixing tea saponin with chlorantraniliprole or tolfenpyrad, a pesticide with certain ratio is prepared to control, has the advantages of good effect and low dosage, and can delay the resistance ofto pesticides.

Pesticide composition for controlling Spodoptera frugiperda

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Hefei, China

Xianyan Su