Jiangsu Academy of Agricultural Sciences

Jiangsu Academy of Agricultural Sciences Institute of Food Safety and Monitoring Technology

Jiangsu Academy of Agricuultural Sciences

Xiangyang Yu

Xiao Zhang

Yuan Liu

Xianjin Liu

Yajing Xie

Chongxin Xu

Cunzheng Zhang

Donglan Wang

Yixin Li

Ruohan Wu

Qun Wan

Jinjin Cheng

Ning Zhu

Gary Banuelos

Hongmei Jin

Yanyun Zhu

Yiwei Dong

AnYAN2 having high-concentration selenite tolerance, the preservation number thereof being GDMCC No. 61594, and a method for fermenting inorganic selenium into nano-selenium by using the strain are provided. The obtained nano-selenium can promote the growth of leaf vegetables, increase the content of chlorophyll, Vc, and total phenol, etc, reduce the content of nitrate, and improve the quality of leaf vegetables. In addition, the present application further provides a nano-selenium liquid fertilizer prepared by fermentation using the strain.

and application thereof in synthesis of nano-selenium

The disclosure discloses a Chinese cabbage plasma membrane intrinsic aquaporin, having an amino acid sequence as set forth in SEQ ID NO. 2, and the nucleotide sequence of the encoding gene BraPIP2;1 thereof is as set forth in SEQ ID NO. 1. The plasma membrane intrinsic aquaporin has the characteristic of sensitively responding to neonicotinoid insecticides (thiamethoxam, imidacloprid, etc.) in the external environment. At the same time, it has the function of mediating transmembrane transport of the neonicotinoid insecticides, promoting accumulation of the neonicotinoid insecticides in plant roots and leaves, which has important application value in guiding efficient and simple use of pesticides, development of new systemic pesticides, etc.

Plasma membrane intrinsic aquaporin for absorbing and transporting neonicotinoid insecticides, and coding gene and use therefor

The disclosure discloses a Chinese cabbage plasma membrane intrinsic aquaporin, having an amino acid sequence as set forth in SEQ ID NO. 2, and the nucleotide sequence of the encoding gene BraPIP1;1 thereof is as set forth in SEQ ID NO. 1. The plasma membrane intrinsic aquaporin has the characteristic of sensitively responding to neonicotinoid insecticides (thiamethoxam, imidacloprid, etc.) in the external environment. At the same time, it has the function of mediating transmembrane transport of the neonicotinoid insecticides, promoting accumulation of the neonicotinoid insecticides in plant roots and leaves, which has important application value in guiding efficient and simple use of pesticides, development of new systemic pesticides, etc.

Provided are a human-derived insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and an anti-Cry1B toxin idiotype single-chain antibody encoded by said human-derived insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2. The idiotype single-chain antibody is a &#x3b2;-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has binding activity tomidgut peritrophic membrane specific receptor BBMV.

Human-derived insect-resistant gene and anti-CRY1B toxin idiotype single-chain antibody encoded thereby and application thereof

An anthropogenic pest-resistant gene having a nucleotide sequence as represented by SEQ IN NO. 1, and, an anti-Cry1Ab toxin idiotypic single-chain antibody encoded by the anthropogenic pest-resistant gene and having a nucleotide sequence as represented by SEQ IN NO. 2.

Human-derived insect-resistant gene and anti-Cry1B toxin idiotype single-chain antibody encoded thereby and application thereof

An anthropogenic insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and a Cry1C toxin idiotype single-chain antibody encoded by said anthropogenic insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2; the antibody is a &#x3b2;-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has binding activity tomidgut peritrophic membrane specific receptor BBMV; the &#x3b2;-type Cry1C toxin idiotype single-chain antibody of the present invention is obtained without animal immunization, has a short preparation period and small amino acid sequence, and is suitable for large-scale in vitro production. The present invention is an entirely new insect-resistant gene resource, and has significant implications for decreasing the various safety risks associated with the widescale use of existing Bt toxins, substituting Bt toxins in the biocontrol of agricultural pests, and reducing the use of pesticides.

Anthropogenic insect-resistant gene and Cry1C toxin idiotype single-chain antibody encoded thereby and application thereof

Growing community of inventors

Nanjing, China

Xiangyang Yu