Third Wave Technologies, Inc.

Wu-Po Ma

Hatim T Allawi

Victor I Lyamichev

Bruce P Neri

Michael W Kaiser

James J Schaefer

Natalie E Lyamicheva

The present invention provides novel cleavage agents for the cleavage and modification of nucleic acid. The cleavage agents find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, an endonuclease activity is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Endonuclease-substrate complexes

The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.

Enzymes for the detection of specific nucleic acid sequences

Enzymes for the detection of nucleic acid sequences

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Madison, WI, United States of America

Wu-Po Ma