Invitrogen Corporation

Functional Genetics, Inc.

Life Technologies Corporation

Wu-Bo Li

Joel A Jessee

Gulilat Gebeyehu

David M Schuster

Paul E Nisson

Jiulin Xia

Jhy-Jhu Lin

Michael Scott Kinch

Michael Goldblatt

Jonathan Kuo

Joel Jesse

Hanwen Mao

Ke Weng

Heather Thi Thu Ung-Medoff

Manu Kohli

Zenbework Fesseha

Huosheng Chen

Shaojing Chang

Douty Bamba

David Schuster

A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.

Methods of inhibiting HIV infectivity

The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules

The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methyliimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

Compositions and methods for enhanced synthesis of nucleic acid molecules

The present invention is directed to a method for the identification and isolation of specific genetic sequences from cDNA libraries or samples of genomic DNA derived from the cells, tissues or organs of an organism, particularly a mammal, and most particularly a human (including a human embryo or fetus). In particular, the invention is related to a method whereby a tissue-specific cDNA or a genetic marker, not found in cDNA libraries or genomic DNA samples derived from other tissues from the same or a different organism, may be identified and isolated using an Amplification Fragment Length Polymorphism (AFLP)-based technique. This method has applications in the identification and isolation of cDNA sequences and genomic genetic markers which may be used in a variety of medical, forensic, industrial and plant breeding procedures.

Methods for identification and isolation of specific nucleotide sequences in cDNA and genomic DNA

The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.

Normalized nucleic acid libraries and methods of production thereof

The invention generally concerns the use of amino acid denaturants for denaturing or separating double stranded nucleic acid molecules. More specifically, the present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of haptenylated probes and amino acid denaturants to select the desired molecules and eliminate the undesired library members from a sample. The invention also provides a method in which larger or full-length nucleic acid molecules can be isolated from the subpopulation of desired molecules.

Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence

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Gaithersburg, MD, United States of America

Wu-Bo Li