Unilever Patent Holdings B.v.

Novo Nordisk A/s

Good Humor - Breyers Ice Cream, a Division of Conopco, Inc.

Unilever Plc

Good Humor - Breyer Ice Cream, Division of Conopco, Inc.

Wouter Musters

Johannes M Verbakel

Hein Stam

Jacob B Visser

Ole Hvilsted Olsen

Sven Hastrup

Sven Branner

Johan Haverkamp

Maarten Robert Egmond

Eric Casteleijn

Poul Lindegaard

Nina Eriksen

Maria E Suykerbuyk

Pieter Dirk Van Wassenaar

John William Chapman

Jakob De Vlieg

Jan Maat

Marcellus Johannes Augustinus De Groot

Alida Godelieve Maria Beijersbergen

Robertus Johannes Gouka

Peter J Van De Vonderwoort

Peter J Schaap

Cornelis Antonius Van Den Hondel

Use of a polypeptide or protein with amino acid sequence corresponding substantially to AFP-type III HPLC 12 as additive in a product for improvement of said product, said improvement residing in improved properties of modification of ice crystal growth processes influencing size and shape characteristics of ice in particular in regrowth thereby e.g. minimising potential freezing damage e.g. by preventing or inhibiting ice recrystallisation of the product upon freezing, said use occurring in a manner known per se for anti freeze peptides to obtain higher specific modification activity in particular antifreeze activity than obtainable with the same amount of Winter Flounder AFP.

Recombinant peptide

Use of a polypeptide or protein with amino acid sequence corresponding substantially to AFP-type III HPLC 12 as additive in a product for improvement of said product, said improvement residing in improved properties of modification of ice crystal growth processes influencing size and shape characteristics of ice in particular in regrowth thereby e.g. minimizing potential freezing damage e.g. by preventing or inhibiting ice recrystallization of the product upon freezing, said use occurring in a manner known per se for anti freeze peptides to obtain higher specific modification activity in particular antifreeze activity than obtainable with the same amount of Winter Flounder AFP.

Frozen food products comprising anti-freeze protein (AFP) type III HPLC 12

A process for site-directed integration of multiple copies of a gene in a mould is provided, which comprises transforming a mould cell containing in its chromosomal DNA a restriction site for a rare-cutting endonuclease, e.g., I-Scel, preferably introduced at a desired locus, e.g., within a selectable marker gene or in the neighborhood thereof, with a piece of DNA comprising multiple copies of at least one expressible gene comprising at least one structural gene encoding a desired protein, surrounded by two DNA fragments homologous to part of the DNA upstream and downstream, and in the neighborhood, of said restriction site, while during the transformation of the mould the presence of the rare-cutting endonuclease is provided, followed by selecting or screening for a mould cell in which the multiple gene copies of said expressible gene are inserted into the chromosomal DNA of the mould. The piece of DNA can comprise a third DNA fragment completing any disrupted or partially deleted selectable marker gene in the chromosomal DNA. Preferably the mould belongs to the genus Aspergillus, especially to the species . Also provided are a transformed mould obtainable by a process according to the invention, a process for culturing such transformed mould, and a process for producing and optionally secreting a desired protein by culturing such transformed mould under conditions whereby the structural gene encoding said desired protein is expressed, and optionally isolating or concentrating the desired protein.

Process for site-directed integration of multiple copies of a gene in a mould

Process for producing/secreting a protein by a transformed mould using

This invention relates to enzymes, to rDNA techniques applicable for example to their production, to mutated genes, vectors and mutant and transformed microorganisms useful in their production, and to their uses including for example enzymatic detergent and cleaning compositions containing them.

Detergent compositions comprising a modified subtilisin

The invention relates to isolation of an Aspergillus gene encoding rhamnogalacturonase (RG-ase) and the construction of recombinant Aspergillus strains with overexpression of RG-ase. These strains can be used for the commercial production of RG-ase. RG-ase is an important enzyme in processes requiring the degradation and/or modification of pectin or modification of pectin-containing vegetable or plant cell wall material. RG-ase may be used in various applications, including the processing of fruits and vegetables, in the extraction of components from vegetable material or for improving the functionality of pectin or pectin-containing vegetable material, food material or plant cell wall material.

Methods of detecting and isolating a ripening form of a polypeptide

Cloning and expression of DNA encoding a ripening form of a polypeptide

The invention relates to recombinant DNA technology for the production of an enzyme having sulfhydryl oxidase ('SOX') activity. This SOX-enzyme can be used where the oxidation of free sulfhydryl groups (thio compounds) to the corresponding disulfides is desirable. SOX enzyme may be used for treatment of bakery products or for removal of off-flavour from milk or beer.

Subtilisin mutants

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Maassluis, Netherlands

Wouter Musters